Table 2.

The Gradient Elution Program Applied for Analysis

t (min)	B (%)	Flow Rate (µL/min)
0	40	250
0.5	40	250
2	70	250
2.1	90	600
2.5	90	600
2.6	40	600
4.0	40	600
4.1	40	250
4.5	40	250

Abbreviation: B, solvent (acetonitrile).