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. Author manuscript; available in PMC: 2019 Dec 3.
Published in final edited form as: Hematol Oncol. 2017 Feb 20;36(1):307–315. doi: 10.1002/hon.2388

FIGURE 1.

FIGURE 1

Semiquantitative IHC-scoring assessment for BRAF VE1 in pediatric LCH. A semiquantitative IHC-scoring scale (0–3+) was combined with percent staining of neoplastic cell cells (0%−100%) to deduce a composite score (see Methods for additional details). Lenient-scoring criteria considered any cytoplasmic staining of 1+ or greater intensity in any amount of neoplastic tissue (≥1%) as positive. Stringent-scoring criteria required greater than or equal to 2+ staining intensity in greater than or equal to 10% neoplastic cells to be considered positive. Light microscopic images acquired at 400× magnification to highlight 1+ VE1 staining. H&E, hematoxylin and eosin, IHC, immunohistochemistry