TABLE 3.

VE1 IHC in decalcified LCH bone samples (n = 7)

				IHC/MOL Concordance
Decalcified Bone Samples	LCH, % of section	Composite VE1 Score, %	AS-qPCR	Lenient IHC (≥1+, ≥1%)	Stringent IHC (≥2+, ≥10%)
LCH-51	40	0+, 0	NEG	NEG	NEG
LCH-31	80	0+, 0	NEG*	NEG	NEG
LCH-55	30	1+, 10	NEG*	POS-FP	NEG
LCH-6	5	1+, 50	NEG	POS-FP	NEG
LCH-33	30	1+, 20	POS	POS	NEG-FN
LCH-13	<5	1+, 50	POS	POS	NEG-FN
LCH-53	25	2+, 60	POS	POS	POS

The asterisks (*) denote decalcified samples that failed AS-qPCR in FFPE sections. Molecular results for these 2 specimens were obtained from nondecalcified sections from the same specimen.

Abbreviations: AS-qPCR, allele-specific real-time PCR; FN, false-negative; FP, false-positive; IHC, immunohistochemistry; MOL, molecular; NEG, negative; POS, positive.