TABLE 1.

Primers for genotyping and quantitative PCR

Genotyping	Primer sequence (5’ → 3’)	Cycling conditions
mNOD1–8	CTTAGGCATGACTCCCTCCTGTCG	(1) 94°C for 2 mins followed by 95°C for 30 secs (2) 40 cycles of 60°C for 30secs, 72°C for 3 mins (3) 72°C for 5 mins
mNOD1–9	GATCTTCAGCAGTTAATGTGGGAGTG
mNOD1–10	CCATTCAGGCTGCGCAACTGTTG
mNOD2–7	CCGGTGGATGTGGAATGTGTGC
mNOD2–8	CATGCATGTGTACGTGAGTGCAC
mNOD2–9	CACTGACCATATAAAGATACACAGGC
qPCR primers	Primer sequence (5’ → 3’)	Cycling conditions
Actb		(1) 50°C for 2 mins, 95°C for 10 mins (2) 40 cycles of 95°C for 15 secs, 60°C for 1 min (3) 95°C for 15 secs, 60°C for 1 min, 95°C for 15 secs
Il10	F: GCTCTTACTGACTGGCATGAG R: CGCAGCTCTAGGAGCATGTG
Ikba	F: GAAGCCGCTGACCATGGAA R: GATCACAGCCAAGTGGAGTGGA
Irak	F: ACCCTGTCCTCGGAACTTCT R: GACAGGACTTCGTCCATCGT
Nod1	F: TCCCTTGCCTGTGAGCAGAAAGTA R: GTGGGTATGTGCCATGCTTTGCTT
Nod2	F: CACACATGGCCTTTGGTTCCAGT R: AAAGAGCTGCAGTTGAGGGAGGAA
Tnfa	F: CCCTCACACTCAGATCATCTTCT R: GCTACGACGTGGGCTACAG