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(A) Coomassie-stained SDS-PAGE of a TS tag pulldown of TRRAP 2033–2088 mixed with MYC 1–190 and MYC 1–190 ΔMB2 at 50 μM each. This result demonstrates that MYC 1–190 and TRRAP 2033–2088 do not interact when mixed in vitro. (B-C) CD spectra of MYC 1–190 and MYC 1–190 ΔMB2 mixed in vitro with TRRAP 2033–2088 at 10 μM each. CD spectra show no gain in secondary structure after mixing either MYC 1–190 or MYC 1–190 ΔMB2 with TRRAP 2033–2088.
