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. 2019 Nov 18;13(11):e0007894. doi: 10.1371/journal.pntd.0007894

Fig 1. Screening of the fragment library.

Fig 1

A. Vero cells were cultured in the presence of compounds for two days. The cytotoxicity of each compound was evaluated with the WST-1 assay reagent. The absorbance of samples was measured using a microplate reader at 450 nm. The reference wavelength was 655 nm. The results shown are the mean and standard deviation of triplicate measurements. B. Vero cells were infected with the DENV2 16681 strain in the presence of compounds. The antiviral effect of each compound was evaluated by measuring the viral RNA in the culture supernatant at 72 hours after infection. The drug sensitivity is displayed as the relative infectivity normalized to control cells without drug treatment. The results shown are the mean and standard deviation of triplicate measurements. C. Vero cells were infected with either the DENV-2 16681 or P04/08 strain in the presence of RK-0404678. The antiviral effect of each compound was evaluated by measuring the viral RNA in the culture supernatant at 72 hours after infection. The drug sensitivity is displayed as the relative infectivity normalized to control cells without drug treatment. The results shown are the mean and standard deviation of triplicate measurements. D. Chemical structure of RK-0404678.