. 2019 Nov 18;13(11):e0007894. doi: 10.1371/journal.pntd.0007894

Table 1. Inhibition of the RdRp activity by RK-0404678 for the full-length DENV1-4 NS5 proteins (determined as IC₅₀) and inhibition of the viral replication of DENV1-4 by RK-0404678 (determined as EC₅₀).

	DENV1	DENV2	DENV3	DENV4
IC₅₀ (μM) Polymerase assay	46.2 ± 2.8	201 ± 4.9	287 ± 11	445 ± 23
EC₅₀ (μM) Cell-based assay	29.5 ± 4.2	6.0 ± 0.30	29.4 ± 1.8	31.9 ± 2.8

The results shown are the mean and standard deviation of quadruplicate measurements for IC₅₀ values in a single experiment and triplicate measurements for EC₅₀ values. For EC₅₀ values, one representative result of three independent experiments is shown. The IC₅₀ and EC₅₀ values were compared by Extra Sum-of-Squares F test in PRISM version 6 (GraphPad Software, Inc.). The p-values for differences in IC₅₀ values were p < 0.0001 for all combinations of the four serotypes. The p-values for differences in EC₅₀ values are as follows: p < 0.0001 for DENV1–DENV2, DENV2–DENV3, and DENV2–DENV4; p = 0.135 for DENV1–DENV3; p = 0.131 for DENV1–DENV4; p = 0.294 for DENV3–DENV4.

Table 1. Inhibition of the RdRp activity by RK-0404678 for the full-length DENV1-4 NS5 proteins (determined as IC50) and inhibition of the viral replication of DENV1-4 by RK-0404678 (determined as EC50).

Table 1. Inhibition of the RdRp activity by RK-0404678 for the full-length DENV1-4 NS5 proteins (determined as IC₅₀) and inhibition of the viral replication of DENV1-4 by RK-0404678 (determined as EC₅₀).