Figure 1. a. Haematoxylin-eosin staining (defect group). Inflammatory cell infiltration in the connective tissue (yellow arrows), increase in osteoclast cells, congestion with dilatation of blood vessels (red arrow), decreased numbers of osteoblast and osteocyte cells, and degeneration of connective tissue fibres. b. Haematoxylin-eosin staining (defect and graft group). Mitotic activity in the osteoblast cells at the periphery of the calvarial bone (blue arrows) and decrease in inflammatory cells between the defect and graft site with a reduction of osteoclast cells (green arrow). 1c. Haematoxylin-eosin staining (defect, graft, and Ganoderma lucidum treated group). Increase in bone matrix and bone trabeculae and increase in osteoblast cells in the calvarial bone periphery (yellow arrows), maturation in osteocyte cells (green arrow), and new bone formation in osteon structures within the bone trabeculae. Scale bar=50 μm.