Figure 2. a. Osteonectin immunostaining (defect group). Positive osteonectin expression in degenerated collagen fibres, inflammatory cells, and osteoclast cells in the defect area (yellow arrows), and negative osteonectin expression in osteoblast cells. b. Osteonectin immunostaining (defect and graft group). Positive osteonectin expression in osteoblasts (yellow arrows) and osteocytes in the graft area. c. Osteonectin immunostaining (defect, graft, and Ganoderma lucidum treated group). Positive osteonectin expression in osteoblasts, osteocytes, and new bone trabeculae (yellow arrows), increased in number along with calvarial bone, and constriction of graft areas. Scale bar=50 μm.