Figure 3. a. Osteopontin immunostaining (defect group). An increase in osteopontin expression in osteoclast cells and inflammatory cells around enlarged blood vessels (yellow arrows). b. Osteopontin immunostaining (defect and graft group). Positive osteopontin expression in osteoblasts and osteocytes at the periphery of calvarial bone (yellow arrows) trabeculae and new bone trabeculae within the graft area. c. Osteopontin immunostaining (defect, graft, and Ganoderma lucidum treated group). Positive osteopontin expression in osteoblasts, osteocytes (yellow arrows), and osteons with osteoblastic activity. Scale bar=50 μm.
