Skip to main content
. Author manuscript; available in PMC: 2019 Dec 3.
Published in final edited form as: Methods Mol Biol. 2018;1840:251–281. doi: 10.1007/978-1-4939-8691-0_18

Table 3.

Sequences of qRT-PCR primers used to detect fetal, pro-fibrotic, early activator, and reference genes

Forward Reverse
Fetal genes
ANP GATAGATGAAGGCAGGAAGCCGC AGGATTGGAGCCCAGAGTGGACTAGG
BNP TGTTTCTGCTTTTCCTTTATCTGTC CTCCGACTTTTCTCTTATCAGCTC
AMHC CTGCTGGAGAGGTTATTCCTCG GGAAGAGTGAGCGGCGCATCAAGG
BMHC TGCAAAGGCTCCAGGTCTGAGGGC GCCAACACCAACCTGTCCAAGTTC
Pro-fibrotic genes
COL1A1 TCACCAAACTCAGAAGATGTAGGA GACCAGGAGGACCAGGAAG
COL3A1 ACAGCAGTCCAACGTAGATGAAT TCACAGATTATGTCATCGCAAAG
Early activator genes
Egr-1 CCTATGAGCACCTGACCACA TCGTTTGGCTGGGATAACTC
lex-1 TTATAGGGTCGGTAAGACAGAGTTG GACGGAGTGTTACCCCTAATCTTAT
c-fos AGCCCCTGTGTACTCCCGTG GCCTTGCCTTCTCTGACTGC
c-jun TTCCTCCAGTCCGAGAGCG TGAGAAGGTCCGAGTTCTTGG
c-myc ATGCCCCTCAACGTGAACTTC GTCGCAGATGAAATAGGGCTG
Reference genes
18S GGAAGGGCACCACCAGGAGT TGCAGCCCCGGACATCTAAG
GAPDH CTCAAGATTGTCAGCAATGCATCC CCAGTGGATGCAGGGATGATGTTC

Primers are in 5’ to 3’ orientation