Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain (Escherichia coli) | MC4100 | (Casadaban, 1976); PMID: 781293 | CGSC#: 6152 | See Supplementary file 1 |
| Strain (Escherichia coli) | NR698 | (Wu et al., 2005); PMID: 15851030 | See Supplementary file 1 | |
| Strain (Escherichia coli) | NR1134 | (Lee et al., 2018); PMID: 29463713 | See Supplementary file 1 | |
| Strain (Escherichia coli) | JCM166 | (Wu et al., 2005); PMID: 15851030 | See Supplementary file 1 | |
| Strain (Escherichia coli) | DEK1 | This paper | See Supplementary file 1 | |
| Strain (Escherichia coli) | DH5α λpir | (Metcalf et al., 1994; Simon et al., 1983) | See Supplementary file 1 | |
| Antibody | Mouse monoclonal Anti-Penta His HRP conjugate | Qiagen | Cat#34460 | (1:5000) |
| Antibody | Mouse monoclonal Anti-FLAG M2 HRP conjugate | Sigma | Cat#A8592; RRID:AB_439702 | (1:50,000) |
| Antibody | Rabbit polyclonal Anti-BamA primary | (Kim et al., 2007); PMID: 17702946 |
(1:5000) | |
| Antibody | Rabbit polyclonal Anti-BamD primary | (Kim et al., 2007); PMID: 17702946 |
(1:5000) | |
| Antibody | Rabbit polyclonal Anti-LptD primary | (Narita et al., 2013); PMID: 24003122 | (1:5000) | |
| Antibody | Rabbit polyclonal Anti-LptE primary | (Chng et al., 2010); PMID: 22936569 |
(1:5000) | |
| Antibody | Rabbit IgG, HRP-linked whole Ab (from donkey) | GE Healthcare | Cat#: NA935 | (1:5000) |
| Recombinant DNA reagent | Plasmids used | This paper | See Supplementary file 2 | |
| Commercial assay | Amersham ECL Western Blotting Detection Kit | GE Healthcare | Cat#:RPN2232 | |
| Chemical compound | p-Benzoylphenylalanine | Bachem | Cat#:4017646 | |
| Chemical compound | 10X Casein blocking buffer | Sigma | Cat#:B6429 | |
| Chemical compound | Anzergent 3–14 | Anatrace | Cat#:AZ314 | |
| Chemical compound | Ni-NTA Superflow | Qiagen | Cat#:30450 | |
| Chemical compound | BMB (1,4-bismaleimidobutane) | ThermoFisher | Cat#:22331 | |
| Chemical compound | L-cysteine hydrochloride | Alfa Aesar | Cat#:L06328 | |
| Chemical compound | TCEP-HCl | VWR | Cat#:97064 | |
| Chemical compound | S35 Methionine | American Radiolabeled Chemicals | Cat#:ARS 104A | |
| Chemical compound | N-ethylmaleimide | Sigma | Cat#:E3876 | |
| Chemical compound | Anti-FLAG M2 magnetic beads | Sigma | Cat#:M8823 | |
| Software | PyMol | Schrodinger |
http://pymol.org; RRID:SCR_000305 |