Table 1. Primers used to generate ISH probes.

T7 or T3 primers were added to the reverse or forward primers of each gene, respectively.

Gene	Forward (5’ → 3’)	Reverse (5’ → 3’)
Dll1	AATTAACCCTCACTAAAGGGAGACTGCTGAGAGAGGAAGGGAG	TAATACGACTCACTATAGGGAGAAGACCCGAAGTGCCTTTGTA
Dll4	AATTAACCCTCACTAAAGGGAGACTACTCAGACACCCAGCTCC	TAATACGACTCACTATAGGGAGAATCCTTTGCAAGCCTCCTCT
Jag1	AATTAACCCTCACTAAAGGGAGACGCCATAGGTAGAGTTTGAGG	TAATACGACTCACTATAGGGAGATAGTTGCTGTGGTTCTGAGC
Jag2	AATTAACCCTCACTAAAGGGAGATGGCACCCAGAACCCTTG	TAATACGACTCACTATAGGGAGAATACTCCGTTGTTTTCCGCC
Ascl1	AATTAACCCTCACTAAAGGGTCGTCCTCTCCGGAACTGAT	TAATACGACTCACTATAGGGAGAAGAAGCAAAGACCGTGGGAG
Upk3a	AATTAACCCTCACTAAAGGGGTGGCTGGACTGTGAACCTC	TAATACGACTCACTATAGGGTTGCCCACCCTGACTAGGTA
Cyp2f2	AATTAACCCTCACTAAAGGGGGAACTTTGGAGGCATGAAA	TAATACGACTCACTATAGGGAACTCCTGAGGCGTCTTGAA