Figure 1.

α5 Integrin expression was upregulated in colorectal adenocarcinoma and largely located in tumor stroma. (A) mRNA expression of ITGA5 in 24 pairs of tumors and matched adjacent normal tissues. Each sample was done in triplicate, and Student’s paired t‐test was used for statistical analysis. (B) IHC staining of α5 in whole‐slide tissue sections of colorectal adenocarcinoma and adjacent normal tissues with two independent anti‐α5 antibodies: a polyclonal antibody (HPA‐00264) or a monoclonal antibody (EPR7854). Magnification: ×100, ×200. Scale bar: 100 μm. (C) Immunofluorescent staining for α5 integrin subunit (red) or β1 integrin subunit (red) with DAPI (blue nuclei) in human adjacent normal tissues. Scale bar: 20 μm.