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Comparison study between the combination of allele‐specific, asymmetric rapid PCR and melting analysis assay and droplet digital PCR for detecting PIK3CA E545K hotspot mutation in early and metastatic BrCa patients
| Combination of allele‐specific, asymmetric rapid PCR and melting analysis assay (Markou et al., 2014) | ddPCR | ||
|---|---|---|---|
| Neg | Neg | Pos | Total |
| 9 | 5 | 14 | |
| Pos | 2 | 21 | 23 |
| Total | 11 | 26 | 37 |
| Concordance: (30/37) 81.8% (P = 0.001, chi‐square test) | |||
