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. 2019 Aug 22;13(12):2574–2587. doi: 10.1002/1878-0261.12562

Figure 2.

Figure 2

miR‐367 silencing inhibits embryonal CNS tumor cell proliferation. (A) Tumor cell growth index along 7 days post‐miR‐367 silencing. See also Fig. S2. (B) Representative immunofluorescence images of tumor cells after EdU incorporation (green) and DAPI staining (gray). Scale bar = 400 µm. (C) Tumor cell proliferation based on positive EdU incorporation, 48 h post‐miR‐367 silencing. Cells with either basal or permanently enhanced OCT4A expression were included in the experiments. Cells transfected with nonspecific oligonucleotides, under the same conditions, were used as controls. Data are expressed as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two‐way ANOVA multiple comparison test).