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. 2019 Aug 22;13(12):2574–2587. doi: 10.1002/1878-0261.12562

Figure 3.

Figure 3

miR‐367 silencing decreases clonogenic potential and neurosphere generation capability of embryonal CNS tumor cells. (A) Representative images of tumor colonies formed by Daoy, USP13‐MED, and USP7‐ATRT cells. Cells were transfected with miR‐367 inhibitor or nonspecific control oligonucleotide and maintained in anchorage‐independent culture conditions until colonies became visible (5 days for Daoy; 12 days for USP13‐MED and USP7‐ATRT). Scale bar = 400 µm. (B) Respective amount of tumor cell colonies generated in each experimental condition. Only colonies larger than 50 μm were counted. (C) Representative images of tumorspheres generated by Daoy, USP13‐MED, and USP7‐ATRT cells transfected with miR‐367 inhibitor or nonspecific control oligonucleotide and kept for 5 days in neurosphere culture medium. Scale bar = 250 µm. (D) Respective amount of tumorspheres generated in each experimental condition. Cells with either basal or permanently enhanced OCT4A expression were included in the experiments. Data are expressed as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two‐way ANOVA multiple comparison test).