Skip to main content
. 2019 Aug 22;13(12):2574–2587. doi: 10.1002/1878-0261.12562

Figure 4.

Figure 4

miR‐367 silencing inhibits embryonal CNS tumor spheroid cell invasion. Tumor cells were transfected with miR‐367 inhibitor or nonspecific control oligonucleotide and embedded in a hydrogel matrix. (A) Representative images of USP13‐MED and USP7‐ATRT spheroids displaying arboreal protrusions formed by invading cells after 1, 4, and 7 days of 3D invasion culture. Scale bar: 400 μm. (B) Kinetics of 3D cell invasion in tumor spheroids of USP13‐MED and USP7‐ATRT cells. Cells with either basal or permanently enhanced OCT4A expression were included in the experiments. Data are expressed as mean ± SEM (*P < 0.05, ****P < 0.0001; two‐way ANOVA with Tukey’s multiple comparison test, nonlinear fit curve test; n = 8 per group).