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. 2019 Aug 22;13(12):2574–2587. doi: 10.1002/1878-0261.12562

Figure 6.

Figure 6

SUZ12 is a direct target of miR‐367. (A) SUZ12 levels in embryonal CNS cells by Western blotting, 48 h post‐miR‐367 silencing. (B) Corresponding densitometry of SUZ12 protein bands. Data are expressed as mean ± SEM (*P < 0.05, **P < 0.01, t test analysis, n = 3 per sample). (C) Reporter sequences of SUZ12 3′UTR carrying Wt or Mut miR‐367 binding sites. (D) Luciferase reporter assay. HEK293 cells were cotransfected with miR‐367 mimic or negative control, and plasmid pSUZ12/3′UTR‐Wt or pSUZ12/3′UTR‐Mut. Relative luciferase activities were measured 48 h after transfection. Results are expressed as percentage mean ± SEM. **P < 0.01, t test compared with respective control condition; n = 3 per group).