Figure 3.

RSG attenuates blood‐brain barrier disruption in tPA‐infused stroke mice. A, BBB leakage was determined by immune‐staining of endogenous mouse IgG in the parenchyma. B, Representative Z‐stack confocal images of the tight junction protein ZO‐1 and endothelial cell marker CD31 in brain sections obtained 1 day after MCAO in mice treated with tPA or RSG + tPA. n = 5‐6 per group. Scale bar = 100μm. C, Quantification of MFI of IgG immunostaining 1 day after stroke in mice treated with tPA or RSG + tPA, n = 6 per group. D, Quantification of endogenous IgG positive area. n = 6 per group. E,F, Correlation between BBB disruption (IgG + area and IgG leakage MFI) and hemorrhagic area in both experimental groups. Data are expressed as mean ± SEM. ** P ≤ .01 vs tPA. RSG, rosiglitazone; tPA, tissue plasminogen activator; MCAO, middle cerebral artery occlusion; BBB, blood‐brain barrier; ZO‐1, Zonula occludens‐1; MFI, mean fluorescent intensity