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. 2019 Nov 18;20(22):5786. doi: 10.3390/ijms20225786

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical analysis of S100A9 in femoral head tissue. Injured vessels (A), marrow adipose (B), fibrotic tissue (C), and osteocytes in cortical bone and inflammatory cells (D), showed strong S100A9 immunostaining, along with significant increases in S100A9-immunostained vessels, fat cells, fibroblasts, osteocytes and inflammatory cells (E). Scale bares, 100 μm (panels 1 and 3) and 50 μm (panels 2 and 4). Data are expressed as mean ± standard errors calculated from 10 patients with ONFH and 6 patients with a femoral neck fracture who required total hip arthroplasty. * p < 0.05.