Table A2.
Primers used in this study.
| GenBank ID | Gene Name | Primer | Purpose |
|---|---|---|---|
| MK111643 | Sc-hep-F | AAGGAGCTGACAAGAGTCAT | Clone the cDNA sequence of Sc-hep |
| Sc-hep-R | GCTGCAACATTTTCTAATAAG | ||
| Sc-hep-qPCR-F | TGCTCACCTTTATTTGCCTTCA | Real-time quantitative PCR | |
| Sc-hep-qPCR-R | CGCTTCTGTCTGTTGTTATACG | ||
| MK605396 | Sc-FPN1-qPCR-F | CAGCCTCACCAGCGTTCTT | |
| Sc-FPN1-qPCR-R | CTCCGACTCTATCACATTCTCCT | ||
| MK605397 | Sc-FTN-qPCR-F | TCCTACACCTTCCTTGCTCTG | |
| Sc-FTN-qPCR-R | GACCACCTCTCCAATCCTCTC | ||
| MK605398 | Sc-TFR1-qPCR-F | GAATGCCTCTGCTGTGTTGAT | |
| Sc-TFR1-qPCR-R | GCCTGTCGTGATGGTCTGA | ||
| AY647430.1 | Sc-IL-1β-qPCR-F | TTCAGTTCAGGAGGACGGATG | |
| Sc-IL-1β-qPCR-R | TGTGGCAAGACAGGTAGAGATT | ||
| JN180845.1 | Sc-IL8-qPCR-F | GAAGAGCAGCAGAGTTATCATCA | |
| Sc-IL8-qPCR-R | ATCTCAGTCTCCTCGCAGTG | ||
| JN969981.1 | Sc-TLR22-qPCR-F | CCTACGCCTACTACTTCTTCTTG | |
| Sc-TLR22-qPCR-R | GGTCTTCCTGCTTCCATAGATG | ||
| MK605399 | Sc-TNF-α-qPCR-F | GCATACACAACCGCACTA | |
| Sc-TNF-α-qPCR-R | CTTCCATTCCAGCACCAA | ||
| KT895250.1 | Sc-SOCS3-qPCR-F | TCCTCACCACACTCCACAAG | |
| Sc-SOCS3-qPCR-F | CACATTGGATACGCAGGTTCTT | ||
| FJ436084 | β-actin-qPCR-F | GTGCGTGACATCAAGGAGAAG | |
| β-actin-qPCR-R | GGAAGGAAGGCTGGAAGAGG | ||
| Sc-hep-met-F1 | TGATGTGATTATATGTTAAGTATTTGTAAATATTA | Methylation | |
| Sc-hep-met-R1 | ACTAATATATTAATACTATATATTTATACAAAC | ||
| Sc-hep-met-F2 | ATTGTAAATGTTTTTTTAGATGTTGTTTTAATAG | ||
| Sc-hep-met-R2 | ATCATCAAACTCAAAAATCTAAATACAAATAATAC | ||
| Sc-hep-DNA-F1 | GGCAGGCTGTAGTTAATGTGT | Clone the DNA sequence of Sc-hep | |
| Sc-hep-DNA-R1 | AGTTCTCCTGACTGATGGTTGA | ||
| Sc-hep-DNA-F2 | CACACTCAACCATCAGTCAGG | ||
| Sc-hep-DNA-R2 | CAGTTTCCCACTACAAATGTTATGC | ||
| Sc-hep-3’-UTR-WT-F | CTCGAGCAGTGTTGCAGTTGCAGTGGCCGT | qPCR analysis of miRNA and Dual-Luciferase reporter assay | |
| Sc-hep-3’-UTR-WT-R | GTCGACAATTTTGCAGGAAACATTAAATGAAC | ||
| Sc-hep-3’-UTR-MT-F | CTCGAGGGAGAGCTCATCATCATCGTCACTGAAGTGCAAGAGCTGGA | ||
| Sc-hep-3’-UTR-MT-R | GTCGACAACCGTACACGAAACATTAAATGAATCACTTCCTGTGTGC | ||
| miR-196b-F | TAGGTAGTTTCAAGTTGTTGGG | ||
| miR-196d-F | TAGGTAGTTTTATGTTGTTGGG | ||
| miR-23b-5p-F | GGGTTCCTGGCGTGCTGATTT | ||
| miR-92b-5p-F | AGGTGTGGGATGTTGTGCAGTGTT | ||
| miR-19c-5p-F | AGTTTTGCAGGATTGCATCCGG | ||
| U6-F | TTGGAACGATACAGAGAAGATTAGCA | ||
| Universe-R | GCTGTCAACGATACGCTCG |