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. 2019 Nov 19;20(22):5806. doi: 10.3390/ijms20225806

Figure 7.

Figure 7

Expression analysis of six wheat native structural genes (TaCHS, TaCHI, TaF3H, TaF3′H, TaDFR, and TaANS) involved in the anthocyanin biosynthesis pathway in the leaves of the three types of stable transgenic wheat lines by quantitative real-time PCR (qRT-PCR). Transcript levels in the leaves of the three types of transgenic lines AL-45 and AL-44 containing ZmC1 and ZmR (yellow columns), AL-30, AL-31, and AL-32 containing ZmC1 only (red columns), AL-40 and AL-41 containing ZmR only (blue columns), as well as the two wild types Fielder (F) and Jingdong18 (JD18) (black columns) were determined in three biological replicates. CK stands for the material used as a reference to calculate the relative expression level. Vertical bars are mean ± SD. Different letters in columns indicate statistically significant differences (p < 0.05).