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. 2019 Nov 26;10:2541. doi: 10.3389/fmicb.2019.02541

Figure 1.

Figure 1

IGF1 expression was upregulated in A549 cells infected with influenza A virus (IAV) PR8. (A) Changes in the level of IGF1 mRNA over time in PR8-infected A549 cells (MOI = 0.5); (B) changes in the level of IGF1 mRNA over MOI in PR8-infected A549 cells at 48 h post-infection; (C) changes in the level of IGF1 protein expression over time in PR8-infected A549 cells (MOI = 0.5); GAPDH was used as loading control, and figures were representative of three independent experiments; (D) changes in the level of IGF1 protein expression over MOI in PR8-infected A549 cells at 48 h post-infection; GAPDH was used as loading control, and figures were representative of three independent experiments; (E) grayscale analysis of Figure 1C, and plotted as the ratio of the integrated density of IGF1/GAPDH; (F) grayscale analysis of Figure 1D, and plotted as the ratio of the integrated density of IGF1/GAPDH. Data were the means ± SD from three independent experiments. **p < 0.01 vs. Mock by t test.