Figure 3.

IGF1 expression is upregulated in BALB/c mice infected with IAV PR8. (A) Changes in the level of IGF1 mRNA over time in the lung tissue of BALB/c mice infected with PR8 (50LD₅₀); (B) changes in the level of IGF1 protein over time in the lung tissue of BALB/c mice infected with PR8 (50LD₅₀); (C) grayscale analysis of Figure 3B, and plotted as the ratio of the integrated density of IGF1/GAPDH; and (D) changes in the level of IGF1 mRNA expression over time in the serum of BALB/c mice infected with PR8 (50LD₅₀). The lung and serum sample were mixed from six mice in the group. Data were the means ± SD from three independent experiments. **p < 0.01 vs. PBS group by t test.