Figure 5.

PPARγ and STAT6 are predicted to be master regulators of macrophage efferocytic function in the poststroke brain. A, Expression profiles of a panel of molecules that promote the resolution of inflammation. B, Expression profiles of a panel of transcription regulators, which affect the efferocytic capacity of phagocytes. Asterisks label the significantly upregulated (red) or downregulated (green) genes in brain macrophages, where ** and *** indicate an adjusted P‐value <.01 and .001, respectively. # indicates the gene product typically inhibits efferocytic capacity. C, Upstream Regulator analyses were performed by IPA on DEGs in macrophages from post‐dMCAO brain versus blood. The activation z‐score and P‐value of overlap were shown for a panel of molecules that are potential upstream regulators. The cutoff values for predicated activation or inhibition were z‐score >2 or <−2 and P‐value <.01. PPARγ and STAT6 were predicted to be upstream regulators that were strongly activated in post‐dMCAO brain macrophages. D, DEGs in the brain macrophages that are regulated by PPARγ and STAT6 are shown in a network view, with annotations on the types of their gene products