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. 2019 Sep 27;3(12):1674–1686. doi: 10.1002/hep4.1433

Figure 5.

Figure 5

Effect of a let7a‐5p mimic and a mimic inhibitor on expression of an ABCC2‐3′ UTR‐luciferase construct expressed in Huh7 cells 48 hours after transfection. (A) Expression of a let7a‐5p mimic significantly repressed luciferase activity. The activity of the mimic was completely reversed by a mimic inhibitor. (B) The effect of the let7a‐5p mimic on luciferase activity was completely abrogated by mutation of the putative let7a‐5p binding site in the ABCC2‐3′ UTR. The miR199a‐5p (C) and miR223‐3p (D) mimics had no effect on the expression of an ABCC2‐3′ UTR‐luciferase construct expressed in Huh7 cells. Each panel represents the mean + SEM of three separate experiments. # P < 0.01.