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. 2019 Nov 10;20(22):5622. doi: 10.3390/ijms20225622

Figure 6.

Figure 6

Expression patterns of proteins involved in signaling pathways after PKM2 knockdown by siRNAs. (A) Effect of PKM2 knockdown on cancer signaling pathways in 786-O cells. Cells were transfected with siRNAs and immunoblotting was performed to evaluate expression levels of signaling pathway proteins. Representative blots are shown. (B) Band intensities were measured and represented as bar graphs relative to GAPDH level. Ratios of p-AKT/AKT, p-mTOR/mTOR, p-GSK3β/GSK3β, and p-p70S6K/p70S6K were measured and depicted as bar graphs. One-way ANOVA was used to compare means of different groups. Differences between means were considered significant at p < 0.05 using Tukey’s multiple comparison test; ** p < 0.01, and *** p < 0.001 as compared with normal control cells. (C) Effect of PKM2 knockdown on expression levels of glycolytic signaling proteins. PKM2 knockdown downregulated expression levels of various glycolytic proteins, thereby reducing glycolysis in PKM2 siRNA-transfected 786-O cells. Representative blots are shown. (D) Band intensities were measured and illustrated as bar graphs relative to GAPDH level. Ratio of p-ERK1/2/ERK1/2 was measured and depicted as a bar graph. One-way ANOVA was used to compare means of different groups. Differences between means were considered significant at p < 0.05 using Tukey’s multiple comparison test; ** p < 0.01, and *** p < 0.001 as compared with normal control cells.