Figure 2.

Comparison of behavior endpoints between the untreated control and C₇₀ NP-exposed zebrafish in 3D locomotion and novel tank tests after 14-day exposure. (A) Average speed, (B) average angular velocity, (C) time in top duration, (D) meandering, (E) freezing time movement ratio, and (F) rapid movement time ratio were analyzed for the 3D locomotion test. For the novel tank test, (G) average speed, (H) freezing time movement ratio, (I) time in top duration, (J) number of entries to the top, (K) latency to enter the top, and (L) total distance traveled in the top were analyzed. The data are expressed as the median with interquartile range. The 3D locomotion test data were analyzed by the Kruskal–Wallis test, with Dunn’s multiple comparisons test as a follow-up test (n = 36 for both control and treatment groups). The novel tank test data were analyzed by two-way ANOVA with Geisser–Greenhouse correction (n = 40 for the untreated control; n = 30 for the 0.5 ppm C₇₀ NP-exposed fish; n = 23 for the 1.5 ppm C₇₀ NPs-exposed fish; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).