Figure A1.

BGN silencing aggravates celastrol-induced cell death in gastric cancer cells. (A) Cells were transfected with scrambled siRNA (con) or siBGN for 72 h prior to celastrol (0.5 μM) treatment for24 h. The efficiency of siBGN in HGC-27 and AGS cells was determined by Western blotting. (B) The MTT assay showed that BGN silencing enhanced celastrol-induced cytotoxicity in HGC-27 and AGS cells. Values were presented as mean ± SD of three determinations obtained from three different experiments, * P < 0.05, ** P < 0.01.