Figure 5.

NAMPT-LMO2 dependent gene expression signature in MOLT4 cells. (A) Schematic of the experimental procedure. Venn diagram (B) and heat map (C) of genes that were significantly differentially regulated upon FK866 treatment compared with DMSO-treated groups of WT LMO2 or K74/78 LMO2 expressing MOLT4 cells (adjusted value P < .05). Genes known to play a role in tumorigenesis are marked in red. (D) Pathway overrepresentation analysis using a list of significantly regulated genes from panel C revealed top significantly enriched signal transduction pathways that are NAMPT-LMO2 dependent. (E) iRegulon transcription factor motif enrichment analysis using a list of significantly upregulated genes from (C) revealed enrichment of genes (normalized enrichment score >3.0 and area under the curve >0.03) with NAMPT-LMO2–specific transcription factor motifs.