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. 2019 Nov 18;20(22):5789. doi: 10.3390/ijms20225789

Figure 5.

Figure 5

Effect of superoxide donors on IgG production, Cx43 expression and p38 activation in cultured spleen cells. (A,B,D,E) Induction of IgG, Cx43 and p38 activation by superoxide donors. Spleen cells isolated from Cx43+/+ mice were stimulated with 2.5 μM menadione or 0.5 μM DMNQ for the indicated time or exposed to the indicated concentrations of the reagents for 24 h. Supernatants and cell lysates were harvested and subjected to Western blot analysis for IgG, Cx43 and p-p38. The quantitation of IgG at 24 h point after treatment with 2.5 μM menadione and 0.5 M DMNQ was shown in (C) and (F), respectively. Data shown are mean ± SE (n = 3). * p < 0.05, ** p < 0.01.