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. 2019 Nov 18;20(22):5789. doi: 10.3390/ijms20225789

Figure 6.

Figure 6

Effects of inhibition of NADPH oxidase (NOX) and p38 on LPS-induced production of IgG. (A) The isolated spleen cells were stimulated with 20 μg/mL LPS in the presence or absence of the indicated concentrations of NOX inhibitors, APO and DPI, for 24 h. Supernatants and cellular proteins were harvested and assayed for IgG, Cx43 and p-p38 using Western blot analysis. The densitometric quantitation of IgG in (A) and (B) are shown in (C) and (D). Data shown are mean ± SE (n = 3). * p < 0.05. (E,F) Effects of p38 inhibitor on LPS-initiated IgG secretion. The isolated spleen cells were treated with 20 μg/mL LPS in the presence or absence of 10 μM SB203580 for 24 h. Supernatants were collected and assayed for IgG. Cellular protein was assayed and used as an internal control. The densitometric quantitation of IgG in (E) is shown in (F). Data shown are mean ± SE (n = 3). * p < 0.05.