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. 2019 Nov 18;12(22):3779. doi: 10.3390/ma12223779

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The analysis of mRNA level of genes associated with apoptosis and cell cycle. The examined targets were: (a) p21, (b) p53, (c) BAX, (d) BCL-2 with (e) BAX/BCL-2 ratio, (f) APAF, (g) Cyclin D, (h) C-MYC, (i) Survivin. An asterisk marks a statistically significant differences determined between tested culture circumstances (* p < 0.05, ** p < 0.01, *** p < 0.001).The expression of genes were measured using the RT-qPCR technique, the relative quantification (RQ) was performed using 2^-DDCt method and scaling to the sample with highest expression of target gene. The results are expressed as mean ± SD.