Figure 3.

Suppression of PCD by m3875. (A) The m3875 suppressed both BAX- and INF1-triggered cell death in Nicotiana benthamiana. The N. benthamiana leaves were infiltrated with inoculation buffer or Agrobacterium tumefaciens cells expressing m3875 or green fluorescent protein (GFP), either alone or followed 24 h later with A. tumefaciens cells carrying the BAX or INF1 gene. The leaves were subjected to decolorization with ethanol and visualized 5 days after BAX- or INF1-infiltration. (B) m3875 inhibited BAX- and INF1-induced H₂O₂ accumulation in N. benthamiana. Infiltration was performed as described above, and the leaves were detached at 2 days after BAX- or INF1-infiltration for 3,3′-diaminobenzidine (DAB) staining.