Figure 4.

Effect of alanine point mutations on m3875-mediated PCD suppression. The charged amino acids, D⁷, R⁹, R¹¹, and K²², within m3875 were individually substituted with alanine that is highlighted in read letter, resulting in A7, A9, A11, A7-11, and A22 (Left panel). Through agroinfiltration, the four m3875 mutants were transiently expressed in the Nicotiana benthamiana leaves 24 h prior to infiltration with A. tumefaciens cells harboring the BAX or INF1 gene, and the photos were taken at 5 days post BAX- or INF1- infiltration (Right panel).