Figure 5.

Characteristics of the PVX-m3875 hybrids in systemically infected Nicotiana benthamiana at 15 days post inoculation (dpi). (A) Systemic symptoms of PVX, PVX-m3875, PVX-A7, PVX-A9, PVX- A11, PVX-A7-11 and PVX-A22. The PVX-infected N. benthamiana plants showed little difference compared with the mock-inoculated N. benthamiana, while the plants infected with the PVX vector expressing m3875 or the mutants developed dwarfing as well as leaf deformation and mosaic. (B) Northern blot analysis of PVX RNA. Total RNA was extracted from the upper systemically infected leaves of a similar developmental stage. Bands that corresponded to the PVX viral RNAs are indicated, and rRNA stained with methylene blue is shown as a loading control. Numbers represent the means with the standard deviation (SD), indicating the relative abundance of the gRNA normalized to that of PVX. The experiment was repeated three times with similar results.