Figure 6.

Effect of m3875 on the regulation of the defense-associated genes in Nicotiana benthamiana. Total RNA was extracted from the leaves that transiently expressed m3875 or GFP at 48 h post inoculation (hpi) and used to measure the relative expression levels of NbCNGC23, NbCNGC24, NbCNGC25, NbCNGC26 (A), NbBI-1, NbBI-2 (B), and NbWRKY9 (C) using RT-qPCR. Three biological replicates (each consisting of three technical replicates) were performed. Expression levels of the indicated genes were individually normalized to the internal reference Nbactin. Bars represent the mean ± standard error, and asterisks denote significant differences according to a Student’s t test (** p < 0.01 and * p < 0.05).