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. 2019 Nov 14;20(22):5706. doi: 10.3390/ijms20225706

Figure 1.

Figure 1

Kras expression by wildtype cells, negative controls and clones in Panc-1, SUIT-2 and TB32047 cell lines. (A) Western blots (WBs) showed similar bands in Panc-1. (B) SUIT-2 negative controls (N; pX) expressed more Kras protein than wildtype (WT) cells and clones. (C) TB32047 clones produced slightly less Kras protein than their WT and NC. No protein expression, indicating KrasG12D knock-out, could be observed in Panc-1, SUIT-2 and TB32047 cell lines. β-actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as loading controls.