Table 4.

LC-MS/MS-based quantitative bacterial phosphoproteome studies.

Bacterium	Year	Phosphoproteins	Phosphorylation Sites	Phosphoproteome	Method	Reference
Bacillus subtilis	2010	27	45	Phosphoproteome changes in different media	SILAC	[15]
Streptomyces coelicolor	2011	127	289	Sporulation factors, transcriptional regulators, protein kinases and other regulatory proteins	Label-free	[17]
E. coli	2013	133	108	Stationary phase	SILAC	[25]
Bacillus subtilis	2014	141	177	Stationary phase	SILAC	[31]
Listeria monocytogenes	2014	191	242	Purine biosynthesis regulated by PrfA phosphorylation	SILAC	[29]
Saccharopolyspora erythraea	2014	88	109	Carbon metabolism, environmental stress and protein synthesis affected by phosphorylation	SRM	[30]
E. coli	2015	71	n.r.	Phosphorylation varied during development	SRM	[34]
Bacillus subtilis	2015	124	155	Spore-specific determinants	Label-free	[35]
Synechocystis sp.	2015	188	262	Increased phosphorylation during nitrogen limitation	Dimethyl	[36]
Acinetobacter baumannii SK17-S	2016	248	410	Antibiotic resistance	Label-free	[6]
Acinetobacter baumannii SK17-R	2016	211	285	Antibiotic resistance	Label-free	[6]
Mycobacterium smegmatis	2017	154	224	Transmembrane proteins	Label-free	[37]
Mycobacterium tuberculosis	2017	257	512	Virulence	Tandem mass tag (TMT)	[38]
Streptomyces coelicolor	2018	48	85	Regulatory proteins	TMT	[40]
Zymomonas mobilis	2019	125	177	N2 fixing regulated by phosphorylation	Label-free	[41]
Streptococcus thermophilus	2019	106	161	Divisome proteins phosphorylated by the PknB kinase	Dimethyl	[42]