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A yeast two-hybrid screen of a human brain cDNA library reveals NS3-interacting host factors. The yeast strain Y2HGold was co-transformed with the prey plasmid AD-host factors and the bait plasmid BD or BD-NS3. Co-transformation with BD/AD, BD-Lamin/AD-T, and BD-p53/AD-T were used as blank, negative, and positive controls, respectively. The screen yielded eight host factors that potentially interact with NS3.
