Figure 4.

Generation and validation of DNAJB6 knockout cells. (A) Illustration of the disrupted alleles of DNAJB6 in HEK293 cells using CRISPR/Cas9. (B) DNAJB6 knockout in cell clones was verified by Western blot, wild type (WT) HEK293 cells are the control. (C) Cell viability assays based on quantitation of ATP. ΔDNAJB6 and parental cells were seeded at 5 × 10³ or 1 × 10⁴ cells per well in 96-well plates in DMEM/10% FBS. Luminescence was recorded 10 min after reagent addition. (Mean ± SD, n = 3, Student’s t test; ns, not significant).