Figure 6.
DNAJB6 inhibits JEV replication but does not affect viral entry. (A) Detection of the expression level of replicon NS3 in ΔDNAJB6 cells and control cells. 2 μg of in vitro-transcribed replicon RNA was transfected into 106 ΔDNAJB6 and parental cells, lysates were collected at the indicated times post transfection and the NS3 expression was analyzed by Western blot. GAPDH was used as an internal control. (B) Luciferase activity of JEV-Rluc-Rep in ΔDNAJB6 and parental cells. Cells were treated as described above; luciferase assays were performed at the indicated time points post transfection (Mean ± SD, n = 3, two-way ANOVA; *** p < 0.001). (C) Role of DNAJB6 in JEV binding and internalization into cells. Virus binding to and internalization into cells were measured by qRT-PCR of JEV RNA (Mean ± SD, n = 3, Student’s t test, ns, not significant).