Figure 7.

JEV infection downregulates the expression of DNAJB6. (A) DNAJB6 mRNA levels in mock and JEV infected (MOI of 1.0) HEK293 cells. The total cellular RNA was extracted and the levels of DNAJB6 were determined by RT-qPCR and normalized to β-actin at each time point. (B-C) DNAJB6 protein levels in mock and JEV infected HEK293 cells. (B) Western blot was performed to examine the expression of the cellular DNAJB6 protein, GAPDH was used as a loading control. (C) Quantitation of DNAJB6 protein normalized to the GAPDH. (Mean ± SD, n = 3, two-way ANOVA; *** p < 0.001).