Table 2.
Plasmids and antibodies used in this study.
| Plasmid and Antibody | Source | Identifier |
|---|---|---|
| pGBKT7-NS3 | Research Center of Swine Disease | N/A |
| pCMV-HA-DNAJB6 | Research Center of Swine Disease | N/A |
| pEGFP-NS3 | Research Center of Swine Disease | N/A |
| SA14/U14163 replicon (Rluc-rep) | [52] | N/A |
| Anti-DNAJB6 antibody [EPR17122]-N-terminal | Abcam | Cat#ab198995 |
| GAPDH polyclonal antibody | ABclonal Technology | Cat#AC001 |
| Monoclonal anti-HA antibody | Sigma Aldrich | Cat#H9658 |
| Alexa Fluor 488-conjugated goat anti-rabbit IgG | Beyotime | Cat#A0423 |
| Alexa Fluor 555-conjugated donkey anti-mouse IgG | Beyotime | Cat#A0460 |
| Mouse and rabbit polyclonal antibodies against JEV Anti-NS3 antibodies | Research Center of Swine Disease | N/A |