Figure 5.

m⁶A status of A5044 differentially regulates the structure of PK7 in human cells. (A) PK7 is a helix that bridges 1548 nts in MALAT1 and was detected by PARIS in HEK293T cells. m⁶A5044 (marked by a caret, ►) was observed by prior (m⁶A/MeRIP-seq) experiments in HEK293T cells [43]. Binding sites for miR-217-5p, miR-101-3p, and the predicted binding site for miR-383-5p were determined from prior experiments [15,16,61,62]. (B) PK7→HP is the predicted structure that forms based on PARIS experiments in HeLa cells [26,27]. The m⁶A5044 mark has not been detected in HeLa cells [43]. All PARS, DMS-seq, and PARIS data are annotated as described in Figure 2.