Figure 7.

Relative RoGalU2 enzyme activity depending on the MgCl₂ and MgSO₄ concentration between 0 mM and 10 mM. Assay mixture contained 2 mM UTP, 2 mM glucose 1-phosphate, 0–10 mM MgCl₂ or MgSO₄, 50 mM Hepes buffer, pH 7.0, 30 µg RoGalU2. Reaction was carried out at 42 °C in 1 mL reaction volume. Means with standard deviations of triplicate measurements are shown. 100% relative RoGalU2 activity correspond to 5.2 U mg⁻¹.