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RoGalU2 enzyme activity depending on the UTP concentration between 10 µM and 5 mM at a fixed glucose 1-phosphate concentration of 250 mM. Fit is calculated by the Model of Yano and Koga [50], for enzyme kinetics with substrate inhibition. The assay mixture contained 0.01–5 mM UTP, 250 mM glucose 1-phosphate, 1 mM MgCl2, 50 mM Hepes buffer, pH 7.4, 1.7 µg RoGalU2. Reaction was carried out at 42 °C in 1 mL volume. Mean values with standard deviations from triplicates are shown.
