Figure 11.
RoGalU2 activity of the improved activity assay compared to an assay with optimal reaction conditions. Reaction solution for improved test contained 1.5 mM UTP, 250 mM glucose 1-phosphate, 1 mM MgCl2, 50 mM Hepes, pH 7.4, 1.7 µg RoGalU2. Reaction was carried out at 42 °C in 1 mL scale. Reaction solution for optimal test contained 1.5 mM UTP, 250 mM glucose 1-P, 1 mM MgSO4, 50 mM Imidazol, pH 7.4, 1.7 µg RoGalU2. Reaction was carried out 43 °C in 1 mL scale. Means with standard deviations are shown (triplicates).