Table 2.

Kinetic properties of UDP-glucose pyrophosphorylases used for the phylogenetic analysis. Units are given in brackets in the table head, unless otherwise indicated.

Organism and Protein	Vmax [U mg−1]		Km [mM]		kcat [s−1]		c [mM]		Ref.
	UTP	G1P	UTP	G1P	UTP	G1P	UTP	G1P
R. opacus 1CP GalU2	177	6.751 1192	0.51	0.91 1502	96.2	3.71 652	0.01–5	0.05–500	This study
E. coli K-12 GalU	340		0.17	0.035	187		2	2	[19]
E. coli K-12 GalF	0.015		0.36	0.52	0.008		2	2	[19]
M. tuberculosis H37Rv GalU	2.5–2.73 9.8 U4	5.8 U4	0.13 0.0124	0.133 0.0454	93.444	55.034	0.001–0.014	0.008–0.054	[51,13]
E. amylovora CFBP 1430 GalU	14.4	32.5	0.027	0.007	7.9	17.2	0.006–0.1	0.003–0.1	[30]
S. coelicolor A3 (2) GalU	270		>10	0.06	149		≤20	n.s.	[10]
B. bifidum PRL2010 GalU	13		0.042	0.098	12		0–2	0–2	[59]
X. capestris pv. campestris str. 8004 GalU	60–90		0.21	0.06	40		n.s.	0-8	[31]
X. axonopodis pv. citris str. 306 GalU	60–90		0.11	n.s.	29		n.s.	n.s.	[31]
S.thermophilus LY03 GalU	0.2		n.s.	n.s.	0.11		1.25	1	[32]

¹ for glucose-1-phosphate concentrations up to 10 mM; ² for glucose-1-phosphate concentrations between 10–500 mM; ³ [51]; ⁴ [13]; n.s.: not specified; The K_m and k_cat values in case of RoGalU2 are apparent values, see Table 1.